Increasing amount of sewage sludge requires their reasonable management, whereas a storage might be environmentally hazardous. Due to organic matter and nutrients presence in sludge, they may be used as organic fertilizers. However, beyond the valuable contests, sewage sludge can also contain toxic or dangerous components. Therefore, there is a need to develop methods for rapid assessment of sediments ecotoxicity, that will determine their possible applicability in agriculture. The Biolog® Ecoplate enables the metabolic profiles diversity evaluation of microbial populations in environmental samples, which reflects the state of their activity. It is regarded as modern technology, that by means of biological properties allows quick characterization of the ecological status of environmental samples, such as sewage sludge.